Consider other
infectious/noninfectious diarrhea causes
and gastrointestinal disease in
immigrants/travelers from endemic areas
Intestinal Amebiasis:
Diagnostic
key: stool examination {routine lab
tests: not useful}
Difficulties in
performing correct stool examinations
Common
false-positives &
false-negatives
False
Negatives caused by:
antimicrobial
agents
soap/tap
water enemas (lyse
trophozoites)
particular
to matter (barium or
bismuth)-obscures
organisms
False
Positives caused by:
leukocytes
misread as amebas
other
amebas confused with E.
histolytica
Should
take multiple stool samples (one
"negative" stool result
is not sufficient to rule out
intestinal amebiasis)
Samples must be
properly maintained if not
analyzed immediately
Presence of many
fecal leukocytes suggests some other
diagnosis since E. histolytica
causes lysis of neutrophils
amebiasis
differentiation from bacterial
diarrheal disease,
e.g.shigellosis
Indirect hemagglutination
test: 70% sensitivity in patients with
active intestinal disease-- only 10%
sensitivity in asymptomatic cyst carrier
Overview:Extraintestinal Amebiasis:-
Differential includes:
pyrogenic abscess
usually
multiple abscesses
associated
with biliary tract
disease or other
intraabdominal disease
metastatic/primary
tumor (includes hemangioma)
Typical amebic abscess:
single
right lobe
Extraintestinal Amebiasis:
Serology helpful if
patient not from an endemic area
Diagnosis made based on:
epidemiological
information
radiographic
findings
ultrasonography-most
cost-effective; slightly
less sensitive than other
techniques
MRI
CT
radioisotope
liver scans
positive serology
most
sensitive: indirect
hemagglutination (95
percent sensitivity in
extraintestinal disease)
-- does not differentiate
present from past
infection
Primary
Reference: Morgan, Juliette and
del Rio, Carlos, Amebiasis in Medicine for the Practicing
Physician (Hurst, J. W., ed) Appleton-Lange, 1996, pp.
457-459.