Leishmania tropica amastigotes from a skin touch preparation. 

In A, a still intact macrophage is practically filled with amastigotes, several of which have clearly visible a nucleus and a kinetoplast (arrows);

In B, amastigotes are being freed from a rupturing macrophage.  Patient with history of travel to Egypt, Africa, and the Middle East. 

Culture in NNN medium followed by isoenzyme analysis identified the species as L. tropica minor. -CDC

"Leishmania mexicana in skin biopsy. 

• Hematoxylin and eosin stain. 

• The amastigotes are lining the wall of two vacuoles, a typical arrangement. 

• The species identification was derived from culture followed by isoenzyme analysis. 

• 26-year old man from Austin, Texas, with a lesion on his left arm."

  • CDC