Anesthesia Pharmacology Chapter 5: Autonomic (ANS) Pharmacology: Introduction
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Autonomic Nervous System: Anatomical Considerations
Figure by Poul-Erik Paulev, M.D., D.Sci, used with permission
Autonomic and Somatic Innervation
Skeletal muscle is innervated by somatic nerves, controlling voluntary actions
All other innervated structures are supplied by the autonomic or involuntary system.
Somatic system: No ganglia present
Autonomic nervous system (ANS) has ganglia.
these ganglia are sites at which preganglionic fibers form synaptic connections with postganglionic neurons
these ganglia are located outside the cerebrospinal axis
Other differences between Somatic and Autonomic Innervation
Motor nerves to skeletal muscle: myelinated
Postganglionic autonomic nerves are nonmyelinated
Denervation of skeletal muscle results in paralysis and atrophy
Denervated smooth muscle or glands retain some activity
First link: Visceral autonomic afferents to the CNS
Non-myelinated, carried to the cerebrospinal axis by autonomic nerves (e.g.vagus and splanchnic)
Some autonomic afferents from skeletal muscle blood vessels and integumental structures may be carried in somatic nerves
Cell bodies of visceral afferents: (a) spinal nerves--in dorsal root ganglia; (b) cranial nerves-- in sensory ganglia
What information gets transmitted?
Mediated Information:
visceral sensation (pain;referred pain)
vasomotor reflexes
respiratory reflexes
viscerosomatic reflexes: Definition: Viscerosomatic: Pertaining to the viscera and body
Figure by Poul-Erik Paulev, M.D., D.Sci, used with permission |
Substance P is an important sensory neurotransmitter, probably especially important in nociception, and is found in:
sensory afferent fibers
dorsal root ganglia
dorsal spinal cord horn
Other agents found in sensory neurons
Somatostatin
Vasoactive intestinal peptide (VIP)
Cholecystokinin (CCK)
Calcitonin gene-related peptide (CGRP) (found with Substance P in cardiovascular sensory nerve fibers)
Dorsal Spinal Cord (substantia gelatinosa) Interneurons
Enkephalins: Antinociceptive due to inhibition of substance P release and reduced transmission to higher centers
CNS and the Autonomic Nervous System
sweating
blood pressure changes
temperature-induced changes in vasomotor tone
emptying of the bowels, bladder, seminal vesicles
Hypothalamic and Nucleus tractus solitarii
Integration of ANS Functions
body temperature regulation
emotions
water balance
sexual response
fat/carbohydrate metabolism
sleep
blood pressure
respiration
Posterior and Lateral Hypothalamic Nuclei are associated with integration of autonomic sympathetic input
Midline nuclei in the region of the Tuber cinereum and anterior nuclei Nuclei are associated with integration of autonomic parasympathetic input
Sympathetic: (Thoracolumbar outflow)
Parasympathetic; (Craniosacral Outflow)
Autonomic Nervous System Neurotransmitters: Summary
Neurotransmitter: Acetylcholine
All preganglionic autonomic fibers
Parasympatheticfibers
A few post-ganglionic sympathetic fibers
Neurotransmitter: Norepinephrine
Postganglionic Sympathetic Fibers
Neurotransmitter: Primary Afferents
Substance P and/or glutamate
Comparisons between Sympathetic and Parasympathetic Nerves
Sympathetic system has a broader distribution, innervating effectors throughout the body
Sympathetic fibers show greater ramification.
Sympathetic preganglionic fibers may traverse through many ganglia before terminiating at its post-ganglionic cell.
Synaptic terminal arborization results in a single preganglionic fiber terminating on many post-ganglionic cells.
This anatomical characteristic is the basis for the diffuse nature of sympathic response in the human and other species.
Parasympathetic system is relatively limited
The parasympathetic system has its terminal ganglia near the end-organ.
Sometimes there is but a one-to-one ratio relationship between pre-and post-ganglionic fibers. The ratio between preganglionic vagal fibers and ganglion cells may be much higher, e.g. 1:8000 for Auerbach's plexus.
Lefkowitz, R.J, Hoffman, B.B and Taylor, P. Neurotrasmission: The Autonomic and Somatic Motor Nervous Systems, In, Goodman and Gillman's The Pharmacologial Basis of Therapeutics,(Hardman, J.G, Limbird, L.E, Molinoff, P.B., Ruddon, R.W, and Gilman, A.G.,eds) TheMcGraw-Hill Companies, Inc.,1996, pp.107
Cell bodies of preganglionic fibers: found in intermediolateral columns of the spinal cord (first thoracic to second or third lumbar segments
Preganglionic fiber axons synapse with sympathetic ganglionic neurons which lie outside the cerebrospinal axis.
Sympathetic ganglia are found at three sites:
Paravertebral
Prevertebral
Terminal
Paraverebral ganglia: 22 interconnected pairs on either sides of the vertebral column. (para: Gr: at the side of along side)
Myelinated preganglionic fibers (white rami: thoracolumbar outflow only) leave through the anterior spinal roots.
Postganglionic fibers (gray rami) runs back to spinal nerves for distribution to:
blood vessesls of the skin
blood vessels of skeletal muscle
sweat glands
pilomotor muscles
Prevertebral Ganglia: abdominal and pelvic location, comprised of:
celiac ganglia
superior mesenteric ganglia
aorticorenal and inferior mesenteric ganglia
Terminal Ganglia: few, residing near the innervated organ, including
ganglia associated with the urinary bladder and rectum
cervical ganglia (neck): three ganglia (chain) mediating vasomotor, secretory, pupillodilatory and pilomotor responses of the head and neck)
All postganglionic fibers arise from cell bodies located within these ganglia; the preganglionic fibers come from upper thoracic segments: No sympathetic preganglionic fibers come from above the first thoracic level
Adrenal medulla is similar to sympathetic ganglia.
Difference:
Epinephrine is released (post-ganglionic sympathetic fibers release norepinephrine)
Chromaffin cells are innerved by preganglionic fibers that release acetylcholine.
Lefkowitz, R.J, Hoffman, B.B and Taylor, P. Neurotrasmission: The Autonomic and Somatic Motor Nervous Systems, In, Goodman and Gillman's The Pharmacologial Basis of Therapeutics, (Hardman, J.G, Limbird, L.E, Molinoff, P.B., Ruddon, R.W, and Gilman, A.G.,eds) TheMcGraw-Hill Companies, Inc.,1996, pp.105-107.
ANS Neurotransmitters: Effector Organs
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Adrenergic |
Effects |
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Cholinergic |
Iris: Radial Muscle |
N.E., alpha-1 receptor |
contraction (mydriasis) |
----- |
Iris: Sphincter muscle |
----- |
----- |
contraction (miosis) |
Ciliary Muscle |
N.E., beta2 receptor |
relaxation (far vision) |
contraction (near vision) |
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Adrenergic |
Effects |
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Cholinergic |
Sino-atrial (SA) Node |
beta1; beta2 |
increase rate |
decrease rate (vagal) |
Atrial muscle |
beta1, beta2 |
increased: contractility, conduction velocity |
decreased: contractility, action potential duration |
Atrio-ventricular (AV) node |
beta1, beta2 |
increased: automaticity*, conduction velocity |
decreased conduction velocity; AV block |
His-Purkinje System |
beta1, beta2 |
increased: automaticity, conduction velocity |
------ |
Ventricles |
beta1, beta2 |
increased: contractility, conduction velocity, automaticity, ectopic pacemaker |
small decrease in contractility |
*An increase in the slope of phase 4 depolarization results in ENHANCED AUTOMATICITY.
As a result of the increase in phase 4 slope the cell reaches threshold more often, with a higher heart rate as a result.
Factors that increase phase 4 depolarization include
mechanical stretch
beta-adrenergic stimulation
hypokalemia
Ischemia can induce abnormal automaticity, i.e. automaticity that occurs in cells not typically exhibiting pacemaker activity.
Acetylcholine is an example of an agent that decreases the slope of phase 4 depolarization and as a result, slows the heart rate.
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Adrenergic |
Effects |
Cholinergic |
Coronary |
alpha1,2; beta2 |
constriction;dilatation |
constriction |
Skin/Mucosa |
alpha 1, 2 |
constriction |
dilatation |
Skeletal Muscle |
alpha; beta2 |
constriction,dilatation |
dilatation |
Cerebral |
alpha1 |
slight constriction |
dilatation |
Pulmonary |
alpha1, beta2 |
constriction; dilatation |
dilatation |
Abdominal viscera |
alpha1, beta2 |
constriction; dilatation |
------- |
Salivary glands |
alpha1,2 |
constriction |
dilatation |
Renal |
alpha 1, 2;beta1,2 |
constriction;dilatation |
--------- |
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Adrenergic Effects |
Cholinergic |
systemic veins |
alpha1,2; beta2 |
constriction; dilatation |
----- |
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Adrenergic Effects |
Cholinergic |
Tracheal and bronchial muscle |
beta2 |
Relaxation |
contraction |
Bronchial glands |
alpha1, beta2 |
decrease secretion; increased secretion |
stimulation |
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Adrenergic Effects |
Cholinergic |
Renin Secretion |
alpha1; beta1 |
decrease; increase |
------- |
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Adrenergic Effects |
Cholinergic |
Pilomotor muscles |
alpha1 |
contraction |
----- |
Sweat glands |
alpha1 |
localized secretion |
generalized secretion |
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Adrenergic Effects |
Cholinergic |
Adrenal medulla |
-- |
---- |
Secretion of epinephrine and norepinephrine (mainly nicotinic and some muscarinic) |
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Adrenergic Effects |
Cholinergic |
Skeletal Muscle |
beta2 |
increased: contractility; |
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Adrenergic Effects |
Cholinergic |
Liver |
alpha1;beta 2 |
glycogenolysis and gluconeogenesis |
-------- |
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Adrenergic Effects |
Cholinergic |
Posterior Pituitary |
beta 1 |
Antidiuretic hormone secretion (ADH) |
------------ |
Based on Table 6-1: Lefkowitz, R.J, Hoffman, B.B and Taylor, P. Neurotransmission: The Autonomic and Somatic Motor Nervous Systems, In, Goodman and Gillman's The Pharmacologial Basis of Therapeutics, (Hardman, J.G, Limbird, L.E, Molinoff, P.B., Ruddon, R.W, and Gilman, A.G.,eds) TheMcGraw-Hill Companies, Inc.,1996, pp.110-111
Characteristics of Autonomic Organ Innervation
Usually, parasympathetic and sympathetic systems are physiological antagonists; that is, if one system facilitates or augments a process the other system inhibits the process.
Since most visceral organs are innervated by both system, the activity of the organ is influenced by both, even though one system may be dominant.
The general pattern of antagonism between sympathetic and parasympathetic systems is not always applicable. The interaction between sympathetic and parasympathetic systems may be independent or interdependent.
Examples of Antagonistic Interactions between Sympathetic and Parasympathetic Systems
Actions of sympathetic and parasympathetic influences on the heart.
Actions of sympathetic and parasympathetic influences on the iris.
Interdependent or Complementary Sympathetic and Parasympathetic Effects
Actions of sympathetic and parasympathetic systems on male sexual organs are complementary.
Independent Effects
Vascular resistance is mainly controlled by sympathetic tone.
Fight or Flight: General Functions of the Autonomic Nervous System
ANS regulates organs/processes not under conscious control including:
circulation
digestion
respiration
temperature
sweating
metabolism
some endocrine gland secretions
Sympathetic system is most active when the body needs to react to changes in the internal or external environment: The requirement for sympathetic activity is most critical for:
temperature regulation
regulation of glucose levels
rapid vascular response to hemorrhage
reacting to oxygen deficiency
During rage or fright the sympathetic system can discharge as a unit--affecting multiorgan systems.
Sympathetic fibers show greater ramification.
Sympathetic preganglionic fibers may traverse through many ganglia before terminiating at its post-ganglionic cell. Synaptic terminal arborization results in a single preganglionic fiber terminating on many post-ganglionic cells.
This anatomical characteristic is the basis for the diffuse nature of sympathic response in the human and other species.
Sympathetic Responses
heart rate increases
blood pressure increases
blood is shunted to skeletal muscles
blood glucose increase
bronchioles dilate
pupils dilate
Parasympathetic responses
slows heart rate
protects retina from excessive light
lowers blood pressure
empties the bowel and bladder
increases gastrointestinal motility
promotes absorption of nutrients
Lefkowitz, R.J, Hoffman, B.B and Taylor, P. Neurotrasmission: The Autonomic and Somatic Motor Nervous Systems, In, Goodman and Gillman's The Pharmacologial Basis of Therapeutics, (Hardman, J.G, Limbird, L.E, Molinoff, P.B., Ruddon, R.W, and Gilman, A.G.,eds) TheMcGraw-Hill Companies, Inc.,1996, pp.108.
Neurotransmitters and the Autonomic Nervous System
Neurotransmitter Criteria: To support the idea that a chemical is a neurotransmitter, several conditions must be satisfied---
The chemical should be found in the appropriate anatomical location (e.g. synaptic terminal)
Enzymes that are involved in "transmitter" synthesis should also be present.
Where possible (as in autonomic transmission), recovery of the "transmitter" in higher quantities following nerve stimulation than in the absence of stimulation.*
Externally applied (e.g. iontophoretically applied) chemical produces the same effect as stimulation. For example, the reversal potential is the same.
Effects of antagonists influence the response to externally applied chemical in the same manner as antagonists modify responses following nerve stimulation.
* may not be possible in many instances
Neurotransmission Steps
Depolarization of the axonal membrane potential results in an action potential.
The upstoke of the action potential is a sodium current flowing through voltage-activated sodium channels
As the membrane potential decreases, activation occurs of an outgoing potassium current, which opposes further depolarization and initiates repolarization.
Longitudinal spread of local depolarizing sodium currents results in progressive, longitudinal activation of sodium channels and new sites of depolarization. The rate of conduction is dependent on the number and synchrony of sodium channel activation.
Number and synchrony of sodium channel activation is membrane potential dependent.
As the resting membrane potential decrease (towards 0), fewer sodium channels will be activated by a depolarizing influence and conduction velocity slows.
In myelinated fibers, depolarization occurs at the Nodes of Ranvier.
Synaptic (Junctional) Activity
Small molecule neurotransmitters (e.g. acetylcholine, norepinephrine) are synthesized at axonal terminals and stored in synaptic vesicles
"The electron micrograph shows synaptic vesicles, purified from rat brain (negative staining, courtesy of Dr. Peter R. Maycox). Each is about 50 nm in diameter (1/20,000th of a millimeter). The inset shows a few vesicles labeled by immunogold for one of the major synaptic vesicle proteins (synaptophysin)."--Research group of Reinhard Jahn
"Synaptic vesicles belong to the most abundant organelles in the body.
The human CNS contains about 1011 neurons. Each of these neuron forms on average about 1000 synapses, and each synapse contains about 500 vesicles, resulting in more than 1017 synaptic vesicles.
This is more than eight magnitudes more than the human genome has base pairs!
Synaptic vesicles can be purified in high yields to high degrees of purity, allowing for their biochemical characterization. Presently, synaptic vesicles are probably the best characterized organelles.
They contain a limited number of proteins that in many cases were discovered as the prototype of small protein families with a widespread distribution on trafficking organelles.
According to our current estimates, the majority of all vesicle-associated proteins are known. The vesicle proteins can be divided into two groups according to their function: the trafficking proteins and the proteins involved in neurotransmitter uptake and storage.
The first group includes proteins of diverse structure such as synaptobrevin/VAMP (involved in exocytotic membrane fusion), synaptotagmin (the exocytotic Ca2+-sensor), rab proteins (probably mediators of protein assembly required for membrane fusion) and several proteins of unknown function that contain four transmembrane domains (synaptophysins, synaptogyrins, SCAMPs).
The second group includes the neurotransmitter transporters, the vacuolar proton ATPase, and probably ion channels required for compensatory charge equilibration." ---Research group of Reinhard Jahn
Isolated neurotransmitter "quanta", perhaps corresponding to single vesicle neurotransmitter quantity, is randomly released in the basal state. This level of release, generating miniature end-plate potentials (mepp's), is necessary for resting skeletal muscle tone.
Action Potentials, promoting calcium influx, induce large, synchronous release of several hundred quanta . Calcium facilitates vesicular membrane-synaptic membrane fusion, resulting in vesicular content discharge into the synaptic cleft.
Many chemical can inhibit norepinephrine or acetylcholine release through receptor interactions at the appropriate terminal. Examples:
Norepinephrine + presynaptic alpha 2-adrenergic receptor (autoreceptor) inhibits norepinephrine release
Alpha2 receptor antagonists increase release of norepinephrine
Neurally-mediated acetylcholine release from cholinergic neurons is inhibited by alpha2-adrenergic receptor agonists
Stimulation of presynaptic beta2 adrenergic receptors increases slightly norepinephrine release
These agents Inhibit neurally-mediated norepinephrine released by interacting with presynaptic receptors
Adenosine
Acetylcholine
Dopamine
Prostaglandins
Enkephalins
Neurotransmitter + Post-Junctional Receptors Interactions Lead to Physiological Response
Neurotransmitter diffuses across the synaptic cleft and bind to post-junctional receptors causing an increase in membrane conductance (ions flow)
Three primary types of changes in conductance may occur:
increase in Na+ (usually) or Ca+ conductance which depolarizes the membrane (EPSP)
Increase in Cl- permeability: inward hyperpolarizing flow : membrane potential more negative) (IPSP)
Increase in K+ permeability; K+ leaves the cells, resulting in hyperpolarization, (IPSP)
If the EPSP is of sufficient magnitude to cause the membrane potential to reach the threshold potential, an action potential results (e.g. in skeletal or cardiac muscle). In gland cells an EPSP may cause secretion; in other cells, an EPSP may increase the rate of spontaneous depolarization.
An IPSP (produced in neurons and smooth, but not skeletal muscle) opposes EPSPs.
Definitions: EPSP: excitatory postsynaptic potential; IPSP: inhibitory postsynaptic potential
Termination of Transmitter Action
Cholinergic: Termination of action of acetylcholine is acetylcholine hydrolysis. (acetylcholinesterase-catalazed)
If acetylcholinesterase is inhibited, the duration of cholinergic effect is increased.
Adrenergic: Termination of action of adrenergic neurotransmitters is by reuptake and diffusion away from receptors.
Amino Acids: Termination of action of amino-acid neurotransmitters is by active transport into neurons and glia
Other Nonelectrogenic Functions
Basal, quantal release of transmitter in quantities insufficient to generate an EPSP may have other actions. These effects may include:
regulation of neurotransmitter biosynthetic and degradative enzymes
pre- and post-synaptic receptor density
Transmitter Synthesis and Degradation
Acetylcholine is synthesized from the immediate precursors acetyl coenzyme A and choline in a reaction catalyzed by choline acetyltransferase (choline acetylase).
Rapid inactivation of acetylcholine is mediated by acetylcholinesterase.
Acetylcholinesterase is present at ganglia, visceral neuroeffector junctions, and neuromuscular junctional endplates.
Another type of cholinesterase, called pseudo-cholinesterase or butyrylcholinesterase has limited presence in neurons, but is present in glia. Most pseudocholinesterase activity is found in plasma and liver.
Pharmacological effects of anti-cholinesterase drugs are due to inhibition of acetylcholinesterase.
Small random release of acetylcholine-quanta, producing miniature end-plate potentials (mepps) , are released by presynaptic terminals.
These small currents were linked to ACh release since anticholinesterases (neostigmine) increased their effects, while cholinergic receptor antagonist (tubocurarine, a nicotinic receptor blocker) blocked.
Anatomical counterpart to the electrophysiological quanta is the synaptic vesicle.
The model is based on the nicotinic, skeletal neuromusclar junction.
Synchronous exocytotic release of many more quanta, dependent on Ca2+ occur when an action potential reaches the terminal.
Exocytotic release of acetylcholine and other neurotransmitters is inhibited by toxins elaborated by Clostridium botulinum.
Botulism
Botulism is caused by the most potent neurotoxins known. The neurotoxins are produced and liberated by Clostridium botulinum.
C. botulinum, ubiquitously found in soil and marine environments, is a group of gram positive anerobes that form spores.
Eight distinct toxins have been characterized, all but one being neurotoxic.
Botulinum neurotoxin affects cholinergic nerve terminals:
postganglionic parasympathetic endings
neuromuscular junctions
peripheral ganglia
CNS is not involved.
Botulinum neurotoxin prevents acetylcholine release:
binds presynaptically
internalized in vesicular form
released into the cytoplasm
the toxin(s), (zinc endopeptidases) causes proteolysis of components of the neuroexocytosis system.
Cholinergic Transmission: Site Differences
Neurotransmitter: Acetylcholine
Receptor Type: Nicotinic
Sectioning and degeneration of motor and post-ganglionic nerve fibers results in:
an enhanced post-synaptic responsiveness, denervation hypersensitivity.
Denervation hypersensivity in skeletal muscle is due to
increased expression of nicotinic cholinergic receptors
and their spread to regions aways from the endplate.
Neurotransmitter: Acetylcholine
Receptor type: Muscarinic
effector coupled to receptor by a G protein
In smooth muscle and in the cardiac conduction system, intrinsic electrical activity and mechanism activity is present, modifiable by autonomic tone.
Activities include propagated slow waves of depolarization: Examples: intestinal motility and spontaneous depolarizations of cardiac SA nodal pacemakers.
Acetylcholine decreases heart rate by decreases SA nodal pacemaker phase 4 depolarization.
The cardiac action potential associated with HIS-purkinje fibers or ventricular muscle consists of five phases
Phase 0 corresponds to Na+ channel activation.
The maximum upstroke slope of phase 0 is proportional to the sodium current.
Phase 0 slope is related to the conduction velocity in that the more rapid the rate of depolarization the greater the rate of impulse propagation.
Phase 1 corresponds to an early repolarizing K+ current. This current like the Phase 0 sodium current is rapidly inactivated.
Phase 2 is the combination of an inward, depolarizing Ca2+ current balanced by an outward, repolarizing K+ current (delayed rectifier).
Phase 3 is also the combination of Ca2+ and K+ currents.
Phase 3 is repolarizing because the outward (repolarizing) K+ current increases while the inward (depolarizing) Ca2+ current is decreasing.
Phase 4 in normal His-Purkinje and ventricular muscle cells is characterized by a balance between outward Na+ current and inward K+ current. As a result, the membrane potential would normally be flat.
In disease states or for other cell types (SA nodal cells) the membrane potential drifts towards threshold. This phenomenon of spontaneous depolarization is termed automaticity and has an important role in arrthymogenesis.
Rate of phase 4 depolarization is decreased by an increase in K+ conductance--which leads to membrane hyperpolarization (takes longer to reach threshold)
Neurotransmitter: Acetylcholine
Receptor type: Nicotinic
Generally similar to skeletal muscle site: initial depolarization is due to receptor activation. The receptor is a ligand-gated channel.
Choline ester administration results in blood vessel dilatation as a result of effects on prejunctional inhibitory synapses of sympathetic fibers and inhibitory cholinergic (non-innervated receptors).
In isolated blood vessel preparations, acetycholine's vasodilator effects are mediated by activation of muscarinic receptors which cause release of nitric oxide, which produces relaxation.
Nicotinic Receptors
Ligand-gated ion channels
Agonist effects blocked by tubocurarine
Receptor activation results in:
rapid increases of Na+ and Ca2+ conductance
deplorization
excitation
Subtypes based on differing subunit composition: Muscle and Neuronal Classification
Muscarinic Receptors
G-protein coupled receptor system
Slower responses
Agonist effects blocked by atropine
At least five receptor subtypes have been described by molecular cloning. Variants have distinct anatomical locations and differing molecular specificities
Lefkowitz, R.J, Hoffman, B.B and Taylor, P. Neurotransmission: The Autonomic and Somatic Motor Nervous Systems, In, Goodman and Gillman's The Pharmacologial Basis of Therapeutics,(Hardman, J.G, Limbird, L.E, Molinoff, P.B., Ruddon, R.W, and Gilman, A.G.,eds) TheMcGraw-Hill Companies, Inc.,1996, pp.112-137
Adrenergic Neurotransmission: Introduction to the Neurotransmitters
Norepinephrine: transmitter released at most postganglionic sympathetic terminals
Dopamine: major CNS neurotransmitter of mammalian extrapyramidal system and some mesocortical and mesolimbic neurononal pathways.
Epinephrine: most important hormone of the adrenal medulla
Catecholamine Synthesis, Storage, and Release
Aromatic L-amino acid decarboxylase (DOPA decarboxylase)
dopa leads to dopamine
methyldopa leads to a-methyldopamine (converted by dopamine ß hydroxylase to the "false transmitter" alpha-norepinephrine)
5-hydroxy-L-tryptophanleads to5-hydroxytryptamine (5-HT)
Tyrosine Hydroxylase
tyrosine leads to DOPA
rate limiting step in pathway
tyrosine hydroxylase is a substrate for cAMP-dependent and Ca2+ - calmodulin-sensitive protein kinase and protein kinase C
Increased hydroxylase activity is associated with the phosphorylated enzyme
In adrenergically innervated tissue: norepinephrine is localized in post-ganglionic nerve terminals
large dense core vesicles (corresponding to chromaffin granules)
small dense core vesicles (containing norepinephrine, ATP, and membrane-bound dopamine ß-hydroxylase
In the adrenal medulla, catecholamines are localized in chromaffin granules.
The most abundant catecholamine in the adrenal medulla is epinephrine.
The adrenal medulla has two cells types containing catecholamines:
one type contains mainly norepinephrine
the second type contains mainly epinephrine.
Epinephrine-containing cells express cytoplasmic phenylethanolamine-N-methyl transferase, allowing conversion of norepinephrine to epinephrine.
Norepinephrine:
synthesized in granules
diffuses out, is methylated in the cytoplasm to epinephrine
then reenters the chromaffin granules.
About half of dopamine is formed in sympathetic neuronal cytoplasm is actively translocated into dopamine ß-hydroxylase-containing vesicles, where the final step, conversion to norepinephrine occurs.
The remaining dopamine is converted to homovanillic acid.
Regulation of Adrenal Medullary Catecholamine Levels
An important factor controlling the rate of epinephrine synthesis and adrenal medullary epinephrine concentration is glucocorticoid concentration.
Glucocorticoids:
are secreted by the adrenal cortex
are carried by an intra-adrenal portal vascular system to the adrenal medullary chromaffin cells
induce synthesis of phenylethanolamine-N-methytransferase.
also increase levels of tyrosine hydreoxylase and dopamine ß-hydroxylase.
also increase levels of tyrosine hydreoxylase and dopamine ß-hydroxylase.
Stress leads to increased corticotropin leads to increased cortisol, increased epinephrine
Following release from adrenergic nerve endings, termination of norepinephrine effect is mainly due to reuptake into presynaptic terminals.
In tissues with wide synaptic gaps and in blood vessels, the effect of released norepinephrine is ended by:
enzymatic breakdown
diffusion away from receptors
extraneuronal uptake.
Neuronal norepinephrine reuptake requires two systems:
a transport system that translocates norepinephrine from extraneuronal spaces into cytoplasm.
a transport system that translocates norepinephrine from the cytoplasm into vesicles.
Translocation of norepinephrine from extraneuronal spaces (uptake I) into the cytoplasm is blocked by:
Cocaine
Tricyclic antidepressants (e.g. imipramine (Tofranil))
Imipramine (Tofranil) : Tricyclic Antidepressant
inhibits norepinephrine and serotonin reuptake
anticholinergic properties
antihistaminic properties
orthostatic hypotension due to alpha receptor blockade
sedation
mild analgesic
Labeled Uses
endogenous depression (an serotonin-specific reuptake inhibitor (SSRI) or other second generation agent is likely to be used first)
occasionally, reactive depression
treatment of enuresis in children older than six.
Shannon, M.T., Wilson, B.A., Stang, C. L. In, Govoni and Hayes 8th Edition: Drugs and Nursing Implications Appleton & Lange, 1995, pp. 616-619
Mechamisms of Indirect Acting Sympathomimetics
An indirect acting sympathomimetic acts mainly by promoting norepinephrine release from nerve terminals.
Mechanism: These amines, all substates for uptake I, act by:
competing with noradrenergic vesicular transport systems, thus making norepinephine more available for release.
Indirect-acting agents, such as tyramine, produce tachyphylaxis in which repetitive doses of tyramine results in a progressively diminishing response.
Tachyphylaxis may result from depletion of a small pool of vesicular norepinphrine residing near the presynaptic membrane.
Uptake II is an extraneuronal (glia, heart, liver, etc )amine translocator that exhibits low affinity for norepinephrine and higher affinities for epinephrine and isoproterenol. This system is of limited physiological significance, unless Uptake I is blocked.
Besides reuptake and diffusion away from receptor sites, catecholamine action can end due to metabolic transformation.
Two primary degradative enzymes:
Monoamine Oxidase (MAO)
Catechol-O-Methyl Transferase (COMT)
Inhibitors of MAO, such as pargyline, phenelzine (Nardil), and tranylcypromine (Parnate) increase norepinephrine, dopamine, and serotonin (5-HT) brain concentrations.
These concentration increases may be responsible for antidepressant action of MAO inhibitors.
Monoamine Oxidase Inhibitor: Phenelzine [Nardil]
Hydrazine MAO inhibitor with amphetamine-like activity
Termination of drug action requires new MAO enzyme synthesis
May cause Hypertensive crisis
Labeled Uses
treatment of endogenous depression
management of depressive phase of bipolar disorder
treatment of severe reactive depression not responsive to other drugs.
Shannon, M.T., Wilson, B.A., Stang, C. L. In, Govoni and Hayes 8th Edition: Drugs and Nursing Implications Appleton & Lange, 1995, pp. 904-905
Catecholamine Release (Adrenal medulla)
Release steps: Chromaffin Granule Adrenal medulla
preganglion fiber releases Ach nicotinic receptor activationdepolarizationCa2+ entry exocytosis of granular content
Ca2+ influx is important in excitation (depolarization)--release coupling
Lefkowitz, R.J, Hoffman, B.B and Taylor, P. Neurotransmission: The Autonomic and Somatic Motor Nervous Systems, In, Goodman and Gillman's The Pharmacologial Basis of Therapeutics,(Hardman, J.G, Limbird, L.E, Molinoff, P.B., Ruddon, R.W, and Gilman, A.G.,eds) TheMcGraw-Hill Companies, Inc.,1996, pp.112-137
Order of agonist potency
Isoproterenol > epinephrine > norepinephrine
ß-receptors are divided into two major categories: ß1 and ß2.
ß1 receptors myocardium.
ß2 receptors smooth muscle and most other sites.
The subdivision of beta receptors followed from the observation that in the heart norepinephrine and epinephrine were equipotent, whereas epinephrine was many fold (10 - 50) more potent at smooth muscle.
A ß3 receptor has been found that is strongly activated by norepinephrine compared to epinephrine and may explain "atypical" pharmacological properties of adipose tissue. The ß3 -receptor is not blocked by propranolol, classified as a non selective beta-receptor blocker.The ß3 -receptor is not blocked by propranolol, classified as a non selective beta-receptor blocker.
Activation of ß1, ß2 and ß3 receptors increases adenylyl cyclase activity (Gs mediated) resulting in a rise of intracellular cAMP.
Cardiac inotropic effects result from increases in Ca2+ concentration, due to:
phosphorylation of L-type Ca2+ channels
phosphorylation of sarcolemmal Ca2+ pumps
direct action Gs action on the L-type channel
Effects on the liver lead to activation of glycogen phosphorylation
ß2 receptor activation mediates relaxation of vascular smooth muscle
ß2 receptor activation mediates relaxation of G.I. smooth muscle. alpha2 adrenergic receptor activation acts presynaptically to reduce Ach release and promote G.I. smooth muscle relaxation. The alpha2 receptor effect is the more important.
Order of agonist potency
epinephrine > norepinephrine >> isoproterenol
Multiple alpha receptor subtypes have been identified.
Multiple forms were suggested when, after administration of an alpha-receptor antagonist, repetitive nerve stimulation resulted in increasing amount of norepinephrine release. This findings suggested a presynaptic alpha-receptor binding site.
Post-synaptic receptors alpha1 .
Pre-synaptic receptors alpha2 .
Alpha2 receptors are also present post-synaptically. This site is involved in the action of some centrally-acting antihypertensive agents, e.g. clonidine.
Some drugs, such as clonidine are more active at alpha2 receptors.
Clonidine (Catapres)
Clonidine acts in the brain at post-synaptic alpha2 receptors, inhibiting adrenergic outflow from the brainstem. Inhibition of sympathetic outflow results in a decrease in blood pressure.
Clonidine reduces cardiac output (by reducing both stroke volume and heart rate) and peripheral resistance. Reduction in stoke volume occurs due to increased venous pooling (decreased preload).
Clonidine does not interfere with cardiovascular responses to exercise.
Renal blood flow and function is maintained during clonidine treatment.
Clonidine has minimal or no effect on plasma lipids.
Adverse Effects
Dry Mouth (xerostomia)
Withdrawal syndrome upon abrupt discontinuation (increased blood pressure, headache, tachycardia, apprehension, tremors)
Bradycardia (in patients with SA nodal abnormality)
Some drugs such as methoxamine (Vasoxyl) or phenylephrine (Neo-Synephrine) are more active at alpha1 receptors.
Multiple forms of both alpha1 and alpha2 receptors have been identified.
D1 receptor activation results in stimulation of adenylyl cyclase activity.
Smooth muscle relaxation (e.g. renal vasodilation) would result from increases in cAMP caused by activation of D1 receptors
Increased cAMP levels may facilitate inactivation of myosin light chain kinase, MLK (activated by calcium-calmodulin complexes which means that increased Ca2+ promotes MLK activation).
Note that only phosphylated myosin can bind to actin and that phosphorylation state is controlled by the enzyme myosin light chain kinase.
D2 receptor activation inhibits cAMP production (inhibits adenylyl cyclase activity), increases K+ conductance and decreases calcium influx.
Following exposure to catecholamines, there is a progressive loss of the ability of the target site to respond to catecholamines. This phenomenon is termed tachyphylaxis, desensitization or refractoriness.
Regulation of catecholamine responsiveness occurs at several levels:
Receptors
G proteins
Adenylyl cyclase
Cyclic nucleotide phosphodiesterase
Stimulation of ß-adrenergic receptors rapidly causes receptor phosphorylation and decreased responsiveness. The phosphorylated receptor exhibits:
decreased coupling to Gs and
decreased stimulation of adenylyl cyclase.
Lefkowitz, R.J, Hoffman, B.B and Taylor, P. Neurotransmission: The Autonomic and Somatic Motor Nervous Systems, In, Goodman and Gillman's The Pharmacologial Basis of Therapeutics,(Hardman, J.G, Limbird, L.E, Molinoff, P.B., Ruddon, R.W, and Gilman, A.G.,eds) TheMcGraw-Hill Companies, Inc.,1996, pp.112-137.Hoffman, B. B. Adrenoceptor-Activating & Other Sympathomimetic Drugs: Introduction to Antimicrobial Agents in Basic and Clinical Pharmacology, (Katzung, B. G., ed) Appleton-Lange, 1998, p.118-122
Other Autonomic Neurotransmitters/Cotransmitters
ATP and catecholamines are found together in neuronal and adrenal medullary storage granules. ATP is released along with transmitters and, in certain cases, has an important role in synaptic transmission.
Vasoactive Intestinal Peptide (VIP)
Vasoactive intestinal peptide (VIP) is found in association with ACh in autonomic parasympathetic fibers innervating blood vessels and exocrine glands and cholinergic sympathetic fibers innervating sweat glands.
VIP may be involved in salivation, tracheal and the GI tract responsiveness to parasympathetic input.
The neuropeptide Y family includes NPY, pancreatic polypeptide, and peptide YY.
NPY in the periphery is associated with sympathetic fibers and assists in maintaining vascular tone.
NPY is a potent, long-lasting vasoconstrictor, especially of small vessels
Purines such as ATP and adenosine may be responsible for apparent non-cholinergic, non-adrenergic autonomic neurotransmission.
Blood vessel endothelium is required for ACh-mediated smooth muscle relaxation.
The endothelial cell layer modulates vessel responsiveness to autonomic and hormonal influences.
Endothelial cell elaborate endothelium-derived relaxing factor (EDRF, nitric oxide) and a contracting factor.
Pharmacological actions of serotonin, histamine, bradykinin, purines, thrombin are mediated to some degree by stimulation of EDRF release.
Endothelial-released nitric oxide diffuses into vascular smooth muscle and activates guanylyl cyclase which increases cGMP.
Clinically, hypotension associated with endotoxemia may be mediated partially by increased release of nitric oxide. A similar mechanism is proposed for hypotension induced by cytokines.
Antatomical Site |
Predominant Autonomic Tone |
Arterioles |
Sympathetic-adrenergic |
Veins |
Sympathetic-adrenergic |
Heart |
Parasympathetic-cholinergic |
Ciliary Muscle |
Parasympathetic-cholinergic |
Gastrointestinal Tract |
Parasympathetic-cholinergic |
Salivary Glands |
Parasympathetic-cholinergic |
Sweat Glands |
Sympathetic-cholinergic |
Taylor, P. Agents Acting at the Neuromuscular Junction and Autonomic Ganglia In, Goodman and Gillman's The Pharmacologial Basis of Therapeutics,(Hardman, J.G, Limbird, L.E, Molinoff, P.B., Ruddon, R.W, and Gilman, A.G.,eds) The McGraw-Hill Companies, Inc.,1996, pp.193-195. Adapted from Table 9-3 |
As pressure rises and especially for rapid increases in pressure:
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Pharmacological Modification of Autonomic Function
Cholinergic
Hemicholinium (HC-3) blocks the choline transport system into the nerve ending, thus limiting acetylcholine (ACh) synthesis.
Adrenergic
Alpha-methyltyrosine inhibits tyrosine hydroxylase thus preventing synthesis of norepinephrine.
Methyldopa inhibits aromatic amino acid decarboxylase and is itself decarboxylated and hydroxylated to form the "false transmitter" alpha-methyl norepinephrine
Cholinergic
Botulinum toxin can be used clinically to treat ocular muscle spasms, muscle dystonias, and spasms.
Botulinus toxin binding at a presynaptic site blocks ACh release.
Vesamicol blocks ACh transport into storage vesicles, thus limiting release.
Adrenergic
Bretylium and guanethidine prevent action-potential mediated norepinephrine release.
Transient release may occur with these agents because they displace norepinephrine from storage sites.
Tyramine, amphetamine, and ephedrine can produce a brief liberation of transmitter.
Reserpine, by inhibiting vesicular uptake, produces a slow, depletion of norepinephrine, ultimately causing adrenergic blockade. Cytoplasmic MAO metabolizes the neurotransmitter.
Reserpine similarly depletes dopamine and serotonin. Physiological effects of reserpine are due to depletion of many transmitters.
Cholinergic
Tetraethylammonium, trimethaphan and hexamethonium are nicotinic ganglionic antagonists.
Decamethonium, a depolarizing drug, selectively causes neuromuscular blockade.
All classes of muscarinic receptors are blocked by atropine.
Adrenergic
Phenylephrine (Neo-Synephrine): an alpha1 receptor agonist.
Clonidine (Catapres): an alpha2 receptor agonist.
Prazosin (Minipress): an example of an alpha1 receptor antagonist.
Yohimbine (Yocon): an example of an alpha2 receptor antagonist.
Isoproterenol (Isuprel): ß1 and ß2 receptor agonist.
Dobutamine (Dobutrex): a relatively selective myocardial ß1 receptor agonist.
Terbutaline (Brethine): relatively selective ß2 receptor agonist.
Propranolol (Inderal): an example of a non-selective beta-adrenergic receptor blocker.
Metoprolol (Lopressor): an example of a relatively selective ß1 receptor antagonist.
Termination of Transmitter Effects: Site 4
Cholinergic
Acetylcholinesterase inhibitors prevent breakdown and inactivation of acetylcholine.
ACh accumulation at the neuromuscular junction causes flacid paralysis.
ACh accumulation at postganglionic muscarinic sites results in either excessive stimulation (contraction & secretion) or inhibition (hyperpolarization), depending on the site.
ACh accumulation at autonomic ganglia cause increased transmission.
Adrenergic
Interference with neurotransmitter reuptake results in potentiation of catecholamine effects.
Cocaine and imipramine are examples of drugs that inhibit the reuptake system.
Monoamine oxidase (MAO) inhibitors potentiate actions of tyramine; whereas catechol-O-methyl transferase (COMT) inhibitors (pyrogallol and tropolone) only slightly increase catecholamine effects.
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