Medical Pharmacology Chapter 35: Antibacterial Drug Practice Questions

Topoisomerase IV S. pneumoniae (Mayer C Aubry A Understanding Tuberculosis - New Approaches to Fighting Against Drug Resistance (2012) https://www.intechopen.com/books/2068
Topoisomerase-primase domain in RED; Breakage reunion domain (BRD): BLUE

Topoisomerase II (DNA gyrase): Homologous to all other type IIA topoisomerases https://en.wikipedia.org/wiki/Type_II_topoisomerase

Quinolones: mechanism/mechanisms of action-
1. Inhibition of bacterial DNA-Gyrase
2. Inhibition of bacterial topoisomerase IV
3. Both
4. Neither
Quinolones: primary target for many gram-positive bacteria:
1. Topoisomerase IV
2. DNA-Gyrase
3. Both
4. Neither
Quinolones: primary target for many gram-negative bacteria:
1. Topoisomerase IV
2. DNA-Gyrase
3. Both
Separates intercalated daughter DNA resulting from DNA replication:
1. DNA-Gyrase
2. Topoisomerase IV
3. Both
4. Neither
Introduces negative supercoils into DNA which decreases supercoiling associated with DNA replication:
1. DNA-Gyrase
2. Topoisomerase IV
Eukaryotic (e.g.mammalian) cells, by contrast to bacteria, do not contain DNA-Gyrase.
1. True
2. False
Eukaryotic (e.g. mammalian cell enzyme) exhibiting a similar mechanism to DNA-Gyrase is type II DNA topoisomerase
1. True
2. False
Quinolones exhibits some capability to inhibit DNA-Gyrase and topoisomerase IV
1. True
2. False
Delafloxacin exhibits a fairly balanced tendency to bind to both DNA-Gyrase and topoisomerase IV:
1. True
2. False
The similar tendency of delafloxacin to bind to both antibacterial target sites (DNA-Gyrase and topoisomerase IV) may explain:
1. Relatively broader spectrum of antibacterial activity
2. Decreased tendency to induce drug resistance
3. Both
4. Neither